Tidy Up   Reverse   Complement

Show code Minimum recognition size for each restriction enzyme 45678 Type of restriction enzyme AllBlunt endsOverhang end Use only this endonuclease Select AarI AasI AatI AatII AccI AccII AccIII Acc16I Acc36I Acc65I AccB1I AccB7I AccBSI AciI AclI AclWI AcoI AcsI AcuI AcvI AcyI AdeI AfaI AfeI AfiI AflII AflIII AgeI AhdI AhlI AjiI AjnI AjuI AleI AlfI AloI AluI AlwI Alw21I Alw26I Alw44I AlwNI Ama87I Aor13HI Aor51HI ApaI ApaLI ApeKI ApoI AscI AseI AsiGI AsiSI AspI Asp700I Asp718I AspA2I AspEI AspLEI AspS9I AssI AsuII AsuC2I AsuHPI AsuNHI AvaI AvaII AviII AvrII AxyI BaeI BalI BamHI BanI BanII BanIII BauI BbeI BbrPI BbsI BbuI BbvI Bbv12I BbvCI BccI BceAI BcgI BciVI BclI BcnI BcuI BdaI BfaI BfiI BfmI BfrI BfuI BfuAI BfuCI BglI BglII BisI BlfI BlnI BlpI BmcAI Bme18I Bme1390I Bme1580I BmeRI BmeT110I BmgBI BmgT120I BmiI BmrI BmrFI BmtI BmuI BoxI BpcI BpiI BplI BpmI BptI Bpu10I Bpu14I Bpu1102I BpuAI BpuEI BpuMI BpvUI BsaI Bsa29I BsaAI BsaBI BsaHI BsaJI BsaMI BsaWI BsaXI Bsc4I Bse1I Bse8I Bse21I Bse118I BseAI BseBI BseCI BseDI Bse3DI BseGI BseJI BseLI BseMI BseMII BseNI BsePI BseRI BseSI BseXI BseX3I BseYI BsgI Bsh1236I Bsh1285I BshFI BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiSI BsiWI BsiYI BslI BslFI BsmI BsmAI BsmBI BsmFI BsnI Bso31I BsoBI Bsp13I Bsp19I Bsp68I Bsp119I Bsp120I Bsp143I Bsp143II Bsp1286I Bsp1407I Bsp1720I BspACI BspANI BspCNI BspDI BspEI BspHI BspLI BspLU11I BspMI BspMAI BspPI BspTI BspT104I BspT107I BspTNI BspXI BsrI BsrBI BsrDI BsrFI BsrGI BsrSI BssAI BssECI BssHII BssKI BssMI BssNI BssNAI BssSI BssT1I Bst6I Bst98I Bst1107I BstACI BstAPI BstAUI BstBI Bst2BI BstBAI Bst4CI BstC8I BstDEI BstDSI BstEII BstENI BstF5I BstFNI BstH2I BstHHI BstKTI BstMAI BstMBI BstMCI BstMWI BstNI BstNSI BstOI BstPI BstPAI BstSCI BstSFI BstSNI BstUI Bst2UI BstV1I BstV2I BstXI BstX2I BstYI BstZI BstZ17I Bsu15I Bsu36I BsuRI BsuTUI BtgI BtgZI BtrI BtsI BtsCI BtuMI BveI Cac8I CaiI CciNI CelII CfoI CfrI Cfr9I Cfr10I Cfr13I Cfr42I ClaI CpoI CseI CspI Csp6I Csp45I CspAI CspCI CviAII CviJI CviKI-1 DdeI DinI DpnI DpnII DraI DraII DraIII DrdI DriI DseDI EaeI EagI Eam1104I Eam1105I EarI EciI Ecl136II EclHKI EclXI Eco24I Eco31I Eco32I Eco47I Eco47III Eco52I Eco57I Eco72I Eco81I Eco88I Eco91I Eco105I Eco130I Eco147I EcoICRI Eco57MI EcoNI EcoO65I EcoO109I EcoP15I EcoRI EcoRII EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI Esp3I FaeI FalI FaqI FatI FauI FauNDI FbaI FblI Fnu4HI FokI FriOI FseI FspI FspAI FspBI Fsp4HI GsuI HaeII HaeIII HapII HgaI HhaI Hin1I Hin1II Hin4I Hin6I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI Hpy8I Hpy99I Hpy188I Hpy188III HpyCH4III HpyCH4IV HpyCH4V HpyF3I HpyF10VI Hsp92I Hsp92II HspAI ItaI KasI KpnI Kpn2I KspI Ksp22I Ksp632I KspAI Kzo9I LguI LweI MabI MaeI MaeII MaeIII MalI MamI MbiI MboI MboII MfeI MflI MhlI MlsI MluI MluNI MlyI Mly113I MmeI MnlI Mph1103I MroI MroNI MroXI MscI MseI MslI MspI Msp20I MspA1I MspCI MspR9I MssI MunI MvaI Mva1269I MvnI MvrI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NmuCI NotI NruI NsbI NsiI NspI NspV OliI PacI PaeI PaeR7I PagI PalAI PasI PauI PceI PciI PciSI PctI PdiI PdmI PfeI Pfl23II PflFI PflMI PfoI PhoI PinAI PleI Ple19I PmaCI PmeI PmlI PpiI PpsI Ppu21I PpuMI PscI PshAI PshBI PsiI Psp5II Psp6I Psp1406I Psp124BI PspCI PspEI PspGI PspLI PspN4I PspOMI PspPPI PspXI PsrI PstI PsuI PsyI PvuI PvuII RcaI RgaI RsaI RsrII Rsr2I SacI SacII SalI SanDI SapI SatI Sau96I Sau3AI SbfI ScaI SchI ScrFI SdaI SduI SexAI SfaNI SfcI SfiI SfoI Sfr274I Sfr303I SfuI SgfI SgrAI SgrBI SgsI SinI SlaI SmaI SmiI SmiMI SmlI SmoI SmuI SnaBI SpeI SphI SrfI Sse9I Sse8387I SseBI SsiI SspI SspBI SstI SstII StrI StuI StyI StyD4I SwaI TaaI TaiI TaqI TaqII TasI TatI TauI TfiI TliI Tru1I Tru9I TseI TsoI Tsp45I Tsp509I TspDTI TspEI TspGWI TspMI TspRI TstI Tth111I Van91I Vha464I VneI VpaK11BI VspI XagI XapI XbaI XceI XcmI XhoI XhoII XmaI XmaCI XmaJI XmiI XmnI XspI ZraI ZrmI Zsp2I Commercial source Only restriction enzymes with known bases (no N,R,Y...) Include Type IIb restriction enzymes (Two cleaves per recognition sequence) Include Type IIs restriction enzymes (Non-palindromic and cleavage outside of the recognition site) When two or more input sequences are searched: Show only endonucleases showing different restriction patterns for searched sequences.

Paste the sequence in the textbox as plain text or fasta (for several sequences).

This service recognizes 249 different cleavage patterns (from all 610 commercially available endonucleases).
Copy and paste PHP script is freely available at biophp.org
This is an open source version of the restriction digest tool originally described by Bikandi et al (2004).
Version: 1.200605045